A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
Report generated on 2021-08-02, 18:19 based on data in:
/g/scb2/zeller/carroll/zru/quast_fastqc
General Statistics
Showing 384/384 rows and 6/7 columns.| Sample Name | N50 (Kbp) | Length (Mbp) | % Dups | % GC | Length | M Seqs |
|---|---|---|---|---|---|---|
| BOV_ANAT_39_02-21-08_R8-0842 | 59.3Kbp | 4.7Mbp | ||||
| BOV_ANAT_39_02-21-08_R8-0842_1 | 15.0% | 52% | 222 bp | 0.5 | ||
| BOV_ANAT_39_02-21-08_R8-0842_2 | 9.6% | 52% | 186 bp | 0.5 | ||
| BOV_ANAT_39_02-21-08_R8-0847 | 77.6Kbp | 4.7Mbp | ||||
| BOV_ANAT_39_02-21-08_R8-0847_1 | 21.3% | 52% | 221 bp | 0.8 | ||
| BOV_ANAT_39_02-21-08_R8-0847_2 | 13.3% | 52% | 182 bp | 0.8 | ||
| BOV_ANAT_56_04-19-08_R8-0968 | 95.6Kbp | 4.9Mbp | ||||
| BOV_ANAT_56_04-19-08_R8-0968_1 | 15.1% | 52% | 228 bp | 0.4 | ||
| BOV_ANAT_56_04-19-08_R8-0968_2 | 10.4% | 52% | 187 bp | 0.4 | ||
| BOV_ANAT_56_04-19-08_R8-0969 | 251.4Kbp | 4.8Mbp | ||||
| BOV_ANAT_56_04-19-08_R8-0969_1 | 29.5% | 51% | 197 bp | 1.8 | ||
| BOV_ANAT_56_04-19-08_R8-0969_2 | 29.0% | 51% | 193 bp | 1.8 | ||
| BOV_CERO_35_10-02-08_R8-2685 | 218.9Kbp | 4.7Mbp | ||||
| BOV_CERO_35_10-02-08_R8-2685_1 | 39.7% | 51% | 195 bp | 1.9 | ||
| BOV_CERO_35_10-02-08_R8-2685_2 | 36.9% | 51% | 185 bp | 1.9 | ||
| BOV_CERO_62_11-12-08_R8-2903 | 268.8Kbp | 4.7Mbp | ||||
| BOV_CERO_62_11-12-08_R8-2903_1 | 39.0% | 52% | 222 bp | 1.9 | ||
| BOV_CERO_62_11-12-08_R8-2903_2 | 37.9% | 52% | 216 bp | 1.9 | ||
| BOV_KENT_14_11-06-07_R6-0942 | 334.9Kbp | 4.7Mbp | ||||
| BOV_KENT_14_11-06-07_R6-0942_1 | 19.7% | 52% | 227 bp | 0.9 | ||
| BOV_KENT_14_11-06-07_R6-0942_2 | 18.8% | 52% | 221 bp | 0.9 | ||
| BOV_KENT_14_12-13-07_R8-0083 | 234.0Kbp | 4.7Mbp | ||||
| BOV_KENT_14_12-13-07_R8-0083_1 | 19.4% | 52% | 227 bp | 0.8 | ||
| BOV_KENT_14_12-13-07_R8-0083_2 | 18.0% | 52% | 222 bp | 0.8 | ||
| BOV_KENT_16_02-13-08_R8-0836 | 301.3Kbp | 4.7Mbp | ||||
| BOV_KENT_16_02-13-08_R8-0836_1 | 19.5% | 52% | 227 bp | 0.9 | ||
| BOV_KENT_16_02-13-08_R8-0836_2 | 17.3% | 52% | 216 bp | 0.9 | ||
| BOV_KENT_16_02-13-08_R8-0838 | 218.9Kbp | 4.7Mbp | ||||
| BOV_KENT_16_02-13-08_R8-0838_1 | 24.4% | 52% | 224 bp | 1.2 | ||
| BOV_KENT_16_02-13-08_R8-0838_2 | 19.5% | 52% | 203 bp | 1.2 | ||
| BOV_KENT_16_04-03-08_R8-0967 | 167.9Kbp | 4.7Mbp | ||||
| BOV_KENT_16_04-03-08_R8-0967_1 | 20.8% | 52% | 227 bp | 1.0 | ||
| BOV_KENT_16_04-03-08_R8-0967_2 | 17.5% | 52% | 208 bp | 1.0 | ||
| BOV_KENT_16_05-21-08_R8-1485 | 484.7Kbp | 4.7Mbp | ||||
| BOV_KENT_16_05-21-08_R8-1485_1 | 33.6% | 51% | 202 bp | 2.1 | ||
| BOV_KENT_16_05-21-08_R8-1485_2 | 33.1% | 51% | 198 bp | 2.1 | ||
| BOV_KENT_17_02-12-08_R8-0823 | 244.9Kbp | 4.7Mbp | ||||
| BOV_KENT_17_02-12-08_R8-0823_1 | 31.3% | 52% | 222 bp | 1.7 | ||
| BOV_KENT_17_02-12-08_R8-0823_2 | 28.3% | 52% | 210 bp | 1.7 | ||
| BOV_KENT_17_11-07-07_R6-0957 | 250.0Kbp | 4.7Mbp | ||||
| BOV_KENT_17_11-07-07_R6-0957_1 | 19.0% | 52% | 224 bp | 0.8 | ||
| BOV_KENT_17_11-07-07_R6-0957_2 | 17.5% | 52% | 215 bp | 0.8 | ||
| BOV_KENT_17_12-12-07_R8-0211 | 161.5Kbp | 4.7Mbp | ||||
| BOV_KENT_17_12-12-07_R8-0211_1 | 20.2% | 52% | 225 bp | 0.9 | ||
| BOV_KENT_17_12-12-07_R8-0211_2 | 17.4% | 52% | 211 bp | 0.9 | ||
| BOV_KENT_19_11-19-07_R6-0966 | 244.9Kbp | 4.7Mbp | ||||
| BOV_KENT_19_11-19-07_R6-0966_1 | 22.6% | 52% | 193 bp | 1.2 | ||
| BOV_KENT_19_11-19-07_R6-0966_2 | 21.5% | 52% | 189 bp | 1.2 | ||
| BOV_KENT_42_03-13-08_R8-0863 | 408.3Kbp | 4.8Mbp | ||||
| BOV_KENT_42_03-13-08_R8-0863_1 | 17.4% | 52% | 228 bp | 0.8 | ||
| BOV_KENT_42_03-13-08_R8-0863_2 | 16.4% | 52% | 221 bp | 0.8 | ||
| BOV_MELA_01_10-02-07_R6-0951 | 244.9Kbp | 4.8Mbp | ||||
| BOV_MELA_01_10-02-07_R6-0951_1 | 18.1% | 52% | 227 bp | 0.8 | ||
| BOV_MELA_01_10-02-07_R6-0951_2 | 17.3% | 52% | 222 bp | 0.8 | ||
| BOV_MELA_01_10-30-07_R6-0802 | 336.5Kbp | 4.8Mbp | ||||
| BOV_MELA_01_10-30-07_R6-0802_1 | 16.2% | 52% | 226 bp | 0.7 | ||
| BOV_MELA_01_10-30-07_R6-0802_2 | 15.2% | 52% | 218 bp | 0.7 | ||
| BOV_MELA_01_10-30-07_R6-0803 | 207.1Kbp | 4.8Mbp | ||||
| BOV_MELA_01_10-30-07_R6-0803_1 | 16.7% | 52% | 225 bp | 0.7 | ||
| BOV_MELA_01_10-30-07_R6-0803_2 | 15.2% | 52% | 214 bp | 0.7 | ||
| BOV_MELA_01_10-30-07_R6-0810 | 208.4Kbp | 4.8Mbp | ||||
| BOV_MELA_01_10-30-07_R6-0810_1 | 17.8% | 52% | 222 bp | 0.8 | ||
| BOV_MELA_01_10-30-07_R6-0810_2 | 17.0% | 52% | 215 bp | 0.8 | ||
| BOV_MELA_01_12-11-07_R8-0198 | 240.4Kbp | 4.8Mbp | ||||
| BOV_MELA_01_12-11-07_R8-0198_1 | 11.7% | 52% | 227 bp | 0.4 | ||
| BOV_MELA_01_12-11-07_R8-0198_2 | 10.3% | 52% | 220 bp | 0.4 | ||
| BOV_NEWP_17_11-07-07_R6-0956 | 172.3Kbp | 5.0Mbp | ||||
| BOV_NEWP_17_11-07-07_R6-0956_1 | 21.9% | 52% | 227 bp | 0.7 | ||
| BOV_NEWP_17_11-07-07_R6-0956_2 | 20.3% | 52% | 217 bp | 0.7 | ||
| BOV_NEWP_17_12-12-07_R8-0206 | 300.2Kbp | 5.0Mbp | ||||
| BOV_NEWP_17_12-12-07_R8-0206_1 | 31.3% | 52% | 223 bp | 1.2 | ||
| BOV_NEWP_17_12-12-07_R8-0206_2 | 27.8% | 52% | 209 bp | 1.2 | ||
| BOV_NEWP_35_10-02-08_R8-2688 | 503.8Kbp | 4.9Mbp | ||||
| BOV_NEWP_35_10-02-08_R8-2688_1 | 30.4% | 50% | 200 bp | 1.5 | ||
| BOV_NEWP_35_10-02-08_R8-2688_2 | 27.7% | 50% | 188 bp | 1.5 | ||
| BOV_NEWP_62_10-14-08_R8-2874 | 496.1Kbp | 4.9Mbp | ||||
| BOV_NEWP_62_10-14-08_R8-2874_1 | 30.8% | 51% | 213 bp | 1.4 | ||
| BOV_NEWP_62_10-14-08_R8-2874_2 | 24.4% | 51% | 189 bp | 1.4 | ||
| BOV_NEWP_62_11-12-08_R8-2900 | 504.2Kbp | 4.9Mbp | ||||
| BOV_NEWP_62_11-12-08_R8-2900_1 | 36.3% | 51% | 218 bp | 2.2 | ||
| BOV_NEWP_62_11-12-08_R8-2900_2 | 35.4% | 51% | 212 bp | 2.2 | ||
| BOV_TYPH_17_12-12-07_R8-0208 | 191.7Kbp | 4.7Mbp | ||||
| BOV_TYPH_17_12-12-07_R8-0208_1 | 25.6% | 52% | 210 bp | 1.3 | ||
| BOV_TYPH_17_12-12-07_R8-0208_2 | 22.5% | 52% | 199 bp | 1.3 | ||
| BOV_TYPH_22_01-18-08_R8-0227 | 214.2Kbp | 4.7Mbp | ||||
| BOV_TYPH_22_01-18-08_R8-0227_1 | 17.9% | 52% | 224 bp | 0.8 | ||
| BOV_TYPH_22_01-18-08_R8-0227_2 | 14.1% | 52% | 202 bp | 0.8 | ||
| BOV_TYPH_22_01-18-08_R8-0228 | 107.9Kbp | 4.7Mbp | ||||
| BOV_TYPH_22_01-18-08_R8-0228_1 | 25.8% | 52% | 224 bp | 1.3 | ||
| BOV_TYPH_22_01-18-08_R8-0228_2 | 22.7% | 52% | 211 bp | 1.3 | ||
| BOV_TYPH_22_01-18-08_R8-0229 | 75.4Kbp | 4.8Mbp | ||||
| BOV_TYPH_22_01-18-08_R8-0229_1 | 23.3% | 52% | 224 bp | 1.1 | ||
| BOV_TYPH_22_01-18-08_R8-0229_2 | 19.6% | 52% | 211 bp | 1.1 | ||
| BOV_TYPH_22_01-18-08_R8-0231 | 95.1Kbp | 4.7Mbp | ||||
| BOV_TYPH_22_01-18-08_R8-0231_1 | 22.5% | 52% | 224 bp | 1.1 | ||
| BOV_TYPH_22_01-18-08_R8-0231_2 | 20.8% | 52% | 217 bp | 1.1 | ||
| BOV_TYPH_22_01-18-08_R8-0271 | 275.5Kbp | 4.7Mbp | ||||
| BOV_TYPH_22_01-18-08_R8-0271_1 | 14.0% | 52% | 220 bp | 0.6 | ||
| BOV_TYPH_22_01-18-08_R8-0271_2 | 12.4% | 52% | 208 bp | 0.6 | ||
| BOV_TYPH_22_01-18-08_R8-0273 | 187.9Kbp | 4.7Mbp | ||||
| BOV_TYPH_22_01-18-08_R8-0273_1 | 30.9% | 52% | 217 bp | 1.8 | ||
| BOV_TYPH_22_01-18-08_R8-0273_2 | 29.1% | 52% | 210 bp | 1.8 | ||
| BOV_TYPH_22_03-14-08_R8-0865 | 216.2Kbp | 4.9Mbp | ||||
| BOV_TYPH_22_03-14-08_R8-0865_1 | 21.1% | 51% | 216 bp | 1.0 | ||
| BOV_TYPH_22_03-14-08_R8-0865_2 | 18.6% | 51% | 203 bp | 1.0 | ||
| BOV_TYPH_25_01-15-08_R8-0233 | 126.2Kbp | 5.0Mbp | ||||
| BOV_TYPH_25_01-15-08_R8-0233_1 | 15.5% | 52% | 230 bp | 0.7 | ||
| BOV_TYPH_25_01-15-08_R8-0233_2 | 13.6% | 52% | 220 bp | 0.7 | ||
| BOV_TYPH_25_11-20-07_R6-0978 | 143.3Kbp | 5.0Mbp | ||||
| BOV_TYPH_25_11-20-07_R6-0978_1 | 27.1% | 52% | 179 bp | 1.8 | ||
| BOV_TYPH_25_11-20-07_R6-0978_2 | 26.0% | 52% | 177 bp | 1.8 | ||
| BOV_TYPH_25_11-20-07_R6-0979 | 266.9Kbp | 4.9Mbp | ||||
| BOV_TYPH_25_11-20-07_R6-0979_1 | 18.8% | 52% | 212 bp | 0.8 | ||
| BOV_TYPH_25_11-20-07_R6-0979_2 | 17.2% | 52% | 205 bp | 0.8 | ||
| BOV_TYPH_25_11-20-07_R6-0983 | 81.8Kbp | 5.0Mbp | ||||
| BOV_TYPH_25_11-20-07_R6-0983_1 | 18.6% | 52% | 220 bp | 0.8 | ||
| BOV_TYPH_25_11-20-07_R6-0983_2 | 17.6% | 52% | 215 bp | 0.8 | ||
| ENV_ANAT_39_01-09-08_R8-0187 | 403.1Kbp | 4.7Mbp | ||||
| ENV_ANAT_39_01-09-08_R8-0187_1 | 23.7% | 51% | 205 bp | 0.9 | ||
| ENV_ANAT_39_01-09-08_R8-0187_2 | 22.8% | 51% | 200 bp | 0.9 | ||
| ENV_ANAT_39_02-21-08_R8-0348 | 209.1Kbp | 4.7Mbp | ||||
| ENV_ANAT_39_02-21-08_R8-0348_1 | 21.9% | 52% | 231 bp | 0.8 | ||
| ENV_ANAT_39_02-21-08_R8-0348_2 | 20.3% | 52% | 221 bp | 0.8 | ||
| ENV_ANAT_39_04-17-08_R8-0905 | 468.8Kbp | 4.7Mbp | ||||
| ENV_ANAT_39_04-17-08_R8-0905_1 | 32.5% | 51% | 200 bp | 1.6 | ||
| ENV_ANAT_39_04-17-08_R8-0905_2 | 31.8% | 51% | 195 bp | 1.6 | ||
| ENV_ANAT_39_11-13-07_R6-0928 | 91.1Kbp | 4.7Mbp | ||||
| ENV_ANAT_39_11-13-07_R6-0928_1 | 17.7% | 52% | 218 bp | 0.7 | ||
| ENV_ANAT_39_11-13-07_R6-0928_2 | 15.4% | 52% | 199 bp | 0.7 | ||
| ENV_ANAT_39_11-13-07_R6-0929 | 240.8Kbp | 4.8Mbp | ||||
| ENV_ANAT_39_11-13-07_R6-0929_1 | 24.8% | 52% | 224 bp | 1.2 | ||
| ENV_ANAT_39_11-13-07_R6-0929_2 | 22.4% | 52% | 210 bp | 1.2 | ||
| ENV_ANAT_39_12-03-08_R8-2860 | 387.1Kbp | 4.7Mbp | ||||
| ENV_ANAT_39_12-03-08_R8-2860_1 | 24.5% | 52% | 239 bp | 0.9 | ||
| ENV_ANAT_39_12-03-08_R8-2860_2 | 20.6% | 52% | 217 bp | 0.9 | ||
| ENV_ANAT_56_02-22-08_R8-0350 | 159.5Kbp | 4.8Mbp | ||||
| ENV_ANAT_56_02-22-08_R8-0350_1 | 18.5% | 52% | 219 bp | 0.8 | ||
| ENV_ANAT_56_02-22-08_R8-0350_2 | 17.4% | 52% | 212 bp | 0.8 | ||
| ENV_ANAT_56_03-25-09_R8-3652 | 169.7Kbp | 4.7Mbp | ||||
| ENV_ANAT_56_03-25-09_R8-3652_1 | 22.6% | 52% | 204 bp | 1.3 | ||
| ENV_ANAT_56_03-25-09_R8-3652_2 | 18.3% | 52% | 184 bp | 1.3 | ||
| ENV_ANAT_56_03-25-09_R8-3653 | 200.0Kbp | 4.8Mbp | ||||
| ENV_ANAT_56_03-25-09_R8-3653_1 | 22.0% | 51% | 207 bp | 1.1 | ||
| ENV_ANAT_56_03-25-09_R8-3653_2 | 13.6% | 51% | 169 bp | 1.1 | ||
| ENV_ANAT_56_04-18-08_R8-0907 | 305.7Kbp | 4.8Mbp | ||||
| ENV_ANAT_56_04-18-08_R8-0907_1 | 27.2% | 51% | 200 bp | 1.7 | ||
| ENV_ANAT_56_04-18-08_R8-0907_2 | 26.9% | 51% | 196 bp | 1.7 | ||
| ENV_ANAT_56_06-12-08_R8-1402 | 415.4Kbp | 4.8Mbp | ||||
| ENV_ANAT_56_06-12-08_R8-1402_1 | 41.5% | 52% | 203 bp | 3.3 | ||
| ENV_ANAT_56_06-12-08_R8-1402_2 | 39.5% | 52% | 195 bp | 3.3 | ||
| ENV_CERO_62_01-22-09_R8-3412 | 196.6Kbp | 4.7Mbp | ||||
| ENV_CERO_62_01-22-09_R8-3412_1 | 19.9% | 52% | 236 bp | 0.7 | ||
| ENV_CERO_62_01-22-09_R8-3412_2 | 18.2% | 52% | 221 bp | 0.7 | ||
| ENV_CERO_62_03-05-09_R8-3445 | 166.9Kbp | 4.7Mbp | ||||
| ENV_CERO_62_03-05-09_R8-3445_1 | 24.3% | 52% | 236 bp | 0.9 | ||
| ENV_CERO_62_03-05-09_R8-3445_2 | 22.9% | 52% | 228 bp | 0.9 | ||
| ENV_CERO_62_04-16-09_R8-3664 | 131.4Kbp | 4.7Mbp | ||||
| ENV_CERO_62_04-16-09_R8-3664_1 | 27.5% | 52% | 236 bp | 1.1 | ||
| ENV_CERO_62_04-16-09_R8-3664_2 | 25.5% | 52% | 225 bp | 1.1 | ||
| ENV_CERO_62_05-28-09_R8-4025 | 69.4Kbp | 4.7Mbp | ||||
| ENV_CERO_62_05-28-09_R8-4025_1 | 25.4% | 53% | 233 bp | 1.0 | ||
| ENV_CERO_62_05-28-09_R8-4025_2 | 24.3% | 53% | 226 bp | 1.0 | ||
| ENV_CERO_62_07-09-09_R8-4030 | 142.8Kbp | 4.7Mbp | ||||
| ENV_CERO_62_07-09-09_R8-4030_1 | 26.8% | 52% | 224 bp | 1.2 | ||
| ENV_CERO_62_07-09-09_R8-4030_2 | 25.5% | 52% | 212 bp | 1.2 | ||
| ENV_CERO_62_07-22-08_R8-1631 | 168.7Kbp | 4.7Mbp | ||||
| ENV_CERO_62_07-22-08_R8-1631_1 | 30.9% | 52% | 178 bp | 1.8 | ||
| ENV_CERO_62_07-22-08_R8-1631_2 | 30.8% | 52% | 175 bp | 1.8 | ||
| ENV_CERO_62_07-22-08_R8-1632 | 213.2Kbp | 4.7Mbp | ||||
| ENV_CERO_62_07-22-08_R8-1632_1 | 31.0% | 52% | 239 bp | 1.4 | ||
| ENV_CERO_62_07-22-08_R8-1632_2 | 29.2% | 52% | 229 bp | 1.4 | ||
| ENV_CERO_62_09-03-08_R8-2026 | 222.6Kbp | 4.7Mbp | ||||
| ENV_CERO_62_09-03-08_R8-2026_1 | 21.4% | 52% | 239 bp | 0.8 | ||
| ENV_CERO_62_09-03-08_R8-2026_2 | 18.8% | 52% | 222 bp | 0.8 | ||
| ENV_CERO_62_10-14-08_R8-2818 | 222.6Kbp | 4.7Mbp | ||||
| ENV_CERO_62_10-14-08_R8-2818_1 | 27.6% | 52% | 236 bp | 1.1 | ||
| ENV_CERO_62_10-14-08_R8-2818_2 | 25.9% | 52% | 226 bp | 1.1 | ||
| ENV_CERO_62_11-12-08_R8-2852 | 210.3Kbp | 4.7Mbp | ||||
| ENV_CERO_62_11-12-08_R8-2852_1 | 20.9% | 52% | 238 bp | 0.7 | ||
| ENV_CERO_62_11-12-08_R8-2852_2 | 18.2% | 52% | 220 bp | 0.7 | ||
| ENV_CERO_62_12-11-08_R8-3138 | 213.4Kbp | 4.7Mbp | ||||
| ENV_CERO_62_12-11-08_R8-3138_1 | 21.0% | 52% | 238 bp | 0.7 | ||
| ENV_CERO_62_12-11-08_R8-3138_2 | 16.0% | 52% | 208 bp | 0.7 | ||
| ENV_KENT_14_02-14-08_R8-0344 | 274.4Kbp | 4.7Mbp | ||||
| ENV_KENT_14_02-14-08_R8-0344_1 | 24.4% | 52% | 211 bp | 1.2 | ||
| ENV_KENT_14_02-14-08_R8-0344_2 | 23.7% | 52% | 207 bp | 1.2 | ||
| ENV_KENT_14_08-30-08_R8-2016 | 247.8Kbp | 4.7Mbp | ||||
| ENV_KENT_14_08-30-08_R8-2016_1 | 24.6% | 52% | 229 bp | 1.2 | ||
| ENV_KENT_14_08-30-08_R8-2016_2 | 15.6% | 52% | 184 bp | 1.2 | ||
| ENV_KENT_14_10-11-07_R6-0798 | 144.5Kbp | 4.7Mbp | ||||
| ENV_KENT_14_10-11-07_R6-0798_1 | 18.8% | 52% | 222 bp | 0.9 | ||
| ENV_KENT_14_10-11-07_R6-0798_2 | 16.6% | 52% | 207 bp | 0.9 | ||
| ENV_KENT_16_09-09-08_R8-2031 | 197.7Kbp | 4.7Mbp | ||||
| ENV_KENT_16_09-09-08_R8-2031_1 | 18.7% | 52% | 229 bp | 0.8 | ||
| ENV_KENT_16_09-09-08_R8-2031_2 | 15.6% | 52% | 210 bp | 0.8 | ||
| ENV_KENT_16_11-11-08_R8-2848 | 247.7Kbp | 4.7Mbp | ||||
| ENV_KENT_16_11-11-08_R8-2848_1 | 19.9% | 52% | 238 bp | 0.8 | ||
| ENV_KENT_16_11-11-08_R8-2848_2 | 17.7% | 52% | 224 bp | 0.8 | ||
| ENV_KENT_16_12-04-07_R8-0061 | 29.8Kbp | 4.7Mbp | ||||
| ENV_KENT_16_12-04-07_R8-0061_1 | 25.6% | 53% | 223 bp | 1.2 | ||
| ENV_KENT_16_12-04-07_R8-0061_2 | 23.7% | 53% | 211 bp | 1.2 | ||
| ENV_KENT_17_03-11-08_R8-0814 | 247.7Kbp | 4.7Mbp | ||||
| ENV_KENT_17_03-11-08_R8-0814_1 | 20.6% | 52% | 221 bp | 0.9 | ||
| ENV_KENT_17_03-11-08_R8-0814_2 | 19.6% | 52% | 216 bp | 0.9 | ||
| ENV_KENT_17_03-11-08_R8-0815 | 76.6Kbp | 4.8Mbp | ||||
| ENV_KENT_17_03-11-08_R8-0815_1 | 16.9% | 52% | 221 bp | 0.8 | ||
| ENV_KENT_17_03-11-08_R8-0815_2 | 13.9% | 52% | 199 bp | 0.8 | ||
| ENV_KENT_17_06-17-08_R8-1405 | 265.7Kbp | 4.7Mbp | ||||
| ENV_KENT_17_06-17-08_R8-1405_1 | 29.5% | 52% | 205 bp | 1.6 | ||
| ENV_KENT_17_06-17-08_R8-1405_2 | 25.9% | 51% | 190 bp | 1.6 | ||
| ENV_KENT_17_07-27-08_R8-1641 | 97.9Kbp | 4.7Mbp | ||||
| ENV_KENT_17_07-27-08_R8-1641_1 | 19.1% | 52% | 232 bp | 0.8 | ||
| ENV_KENT_17_07-27-08_R8-1641_2 | 18.4% | 52% | 227 bp | 0.8 | ||
| ENV_KENT_17_09-10-08_R8-2033 | 89.8Kbp | 4.7Mbp | ||||
| ENV_KENT_17_09-10-08_R8-2033_1 | 19.5% | 52% | 228 bp | 0.9 | ||
| ENV_KENT_17_09-10-08_R8-2033_2 | 15.9% | 52% | 206 bp | 0.9 | ||
| ENV_KENT_17_10-11-07_R6-1000 | 97.8Kbp | 4.7Mbp | ||||
| ENV_KENT_17_10-11-07_R6-1000_1 | 11.8% | 52% | 215 bp | 0.5 | ||
| ENV_KENT_17_10-11-07_R6-1000_2 | 10.1% | 52% | 196 bp | 0.5 | ||
| ENV_KENT_19_01-16-08_R8-0168 | 244.9Kbp | 4.7Mbp | ||||
| ENV_KENT_19_01-16-08_R8-0168_1 | 28.8% | 52% | 201 bp | 1.7 | ||
| ENV_KENT_19_01-16-08_R8-0168_2 | 27.4% | 52% | 193 bp | 1.7 | ||
| ENV_KENT_19_03-13-08_R8-0817 | 107.7Kbp | 4.7Mbp | ||||
| ENV_KENT_19_03-13-08_R8-0817_1 | 19.5% | 52% | 218 bp | 1.0 | ||
| ENV_KENT_19_03-13-08_R8-0817_2 | 13.0% | 52% | 180 bp | 1.0 | ||
| ENV_KENT_19_04-28-08_R8-0916 | 250.1Kbp | 4.7Mbp | ||||
| ENV_KENT_19_04-28-08_R8-0916_1 | 20.1% | 51% | 197 bp | 1.1 | ||
| ENV_KENT_19_04-28-08_R8-0916_2 | 18.1% | 51% | 184 bp | 1.1 | ||
| ENV_KENT_19_04-28-08_R8-0917 | 265.8Kbp | 4.7Mbp | ||||
| ENV_KENT_19_04-28-08_R8-0917_1 | 26.8% | 52% | 205 bp | 1.5 | ||
| ENV_KENT_19_04-28-08_R8-0917_2 | 21.5% | 51% | 182 bp | 1.5 | ||
| ENV_KENT_19_07-21-08_R8-1644 | 205.5Kbp | 4.7Mbp | ||||
| ENV_KENT_19_07-21-08_R8-1644_1 | 20.0% | 52% | 231 bp | 0.9 | ||
| ENV_KENT_19_07-21-08_R8-1644_2 | 17.7% | 52% | 217 bp | 0.9 | ||
| ENV_KENT_19_10-02-08_R8-2813 | 127.9Kbp | 4.7Mbp | ||||
| ENV_KENT_19_10-02-08_R8-2813_1 | 24.8% | 52% | 187 bp | 1.4 | ||
| ENV_KENT_19_10-02-08_R8-2813_2 | 17.5% | 51% | 158 bp | 1.4 | ||
| ENV_KENT_42_01-17-08_R8-0191 | 191.5Kbp | 4.7Mbp | ||||
| ENV_KENT_42_01-17-08_R8-0191_1 | 11.5% | 52% | 227 bp | 0.4 | ||
| ENV_KENT_42_01-17-08_R8-0191_2 | 10.5% | 52% | 217 bp | 0.4 | ||
| ENV_KENT_42_04-23-08_R8-0913 | 433.4Kbp | 4.7Mbp | ||||
| ENV_KENT_42_04-23-08_R8-0913_1 | 27.1% | 51% | 189 bp | 1.7 | ||
| ENV_KENT_42_04-23-08_R8-0913_2 | 26.0% | 51% | 183 bp | 1.7 | ||
| ENV_KENT_42_04-23-08_R8-0914 | 484.6Kbp | 4.7Mbp | ||||
| ENV_KENT_42_04-23-08_R8-0914_1 | 28.8% | 51% | 190 bp | 1.8 | ||
| ENV_KENT_42_04-23-08_R8-0914_2 | 26.1% | 51% | 180 bp | 1.8 | ||
| ENV_KENT_42_07-23-08_R8-1640 | 150.2Kbp | 4.7Mbp | ||||
| ENV_KENT_42_07-23-08_R8-1640_1 | 20.3% | 52% | 231 bp | 0.9 | ||
| ENV_KENT_42_07-23-08_R8-1640_2 | 19.7% | 52% | 226 bp | 0.9 | ||
| ENV_KENT_42_08-19-08_R8-2009 | 197.8Kbp | 4.7Mbp | ||||
| ENV_KENT_42_08-19-08_R8-2009_1 | 21.7% | 52% | 231 bp | 1.0 | ||
| ENV_KENT_42_08-19-08_R8-2009_2 | 16.6% | 52% | 202 bp | 1.0 | ||
| ENV_KENT_42_09-17-08_R8-2236 | 206.4Kbp | 4.7Mbp | ||||
| ENV_KENT_42_09-17-08_R8-2236_1 | 28.0% | 51% | 168 bp | 2.1 | ||
| ENV_KENT_42_09-17-08_R8-2236_2 | 28.4% | 51% | 168 bp | 2.1 | ||
| ENV_KENT_42_11-15-07_R6-0935 | 228.1Kbp | 4.7Mbp | ||||
| ENV_KENT_42_11-15-07_R6-0935_1 | 24.8% | 52% | 222 bp | 1.3 | ||
| ENV_KENT_42_11-15-07_R6-0935_2 | 22.1% | 52% | 205 bp | 1.3 | ||
| ENV_MELA_01_01-10-08_R8-0165 | 211.8Kbp | 4.8Mbp | ||||
| ENV_MELA_01_01-10-08_R8-0165_1 | 23.2% | 52% | 226 bp | 1.2 | ||
| ENV_MELA_01_01-10-08_R8-0165_2 | 21.0% | 52% | 211 bp | 1.2 | ||
| ENV_MELA_01_04-04-08_R8-0901 | 173.8Kbp | 4.8Mbp | ||||
| ENV_MELA_01_04-04-08_R8-0901_1 | 20.3% | 52% | 219 bp | 1.0 | ||
| ENV_MELA_01_04-04-08_R8-0901_2 | 17.6% | 52% | 203 bp | 1.0 | ||
| ENV_MELA_01_05-08-08_R8-1381 | 411.2Kbp | 4.8Mbp | ||||
| ENV_MELA_01_05-08-08_R8-1381_1 | 22.7% | 51% | 192 bp | 1.4 | ||
| ENV_MELA_01_05-08-08_R8-1381_2 | 22.4% | 51% | 188 bp | 1.4 | ||
| ENV_MELA_01_06-13-08_R8-1406 | 492.5Kbp | 4.8Mbp | ||||
| ENV_MELA_01_06-13-08_R8-1406_1 | 34.4% | 52% | 210 bp | 2.2 | ||
| ENV_MELA_01_06-13-08_R8-1406_2 | 33.4% | 52% | 205 bp | 2.2 | ||
| ENV_MELA_01_07-23-08_R8-1633 | 173.0Kbp | 4.8Mbp | ||||
| ENV_MELA_01_07-23-08_R8-1633_1 | 20.5% | 52% | 229 bp | 0.9 | ||
| ENV_MELA_01_07-23-08_R8-1633_2 | 18.4% | 52% | 216 bp | 0.9 | ||
| ENV_MELA_01_09-10-08_R8-2233 | 410.6Kbp | 4.8Mbp | ||||
| ENV_MELA_01_09-10-08_R8-2233_1 | 25.2% | 52% | 231 bp | 1.2 | ||
| ENV_MELA_01_09-10-08_R8-2233_2 | 24.5% | 52% | 227 bp | 1.2 | ||
| ENV_MELA_01_10-02-07_R6-0938 | 217.8Kbp | 4.9Mbp | ||||
| ENV_MELA_01_10-02-07_R6-0938_1 | 23.7% | 52% | 225 bp | 1.2 | ||
| ENV_MELA_01_10-02-07_R6-0938_2 | 22.2% | 52% | 213 bp | 1.2 | ||
| ENV_MELA_01_10-22-08_R8-2829 | 207.1Kbp | 4.8Mbp | ||||
| ENV_MELA_01_10-22-08_R8-2829_1 | 24.6% | 52% | 237 bp | 1.1 | ||
| ENV_MELA_01_10-22-08_R8-2829_2 | 21.3% | 52% | 218 bp | 1.1 | ||
| ENV_MELA_01_NA-NA-NA_R8-0346 | 240.4Kbp | 4.8Mbp | ||||
| ENV_MELA_01_NA-NA-NA_R8-0346_1 | 19.8% | 52% | 213 bp | 0.9 | ||
| ENV_MELA_01_NA-NA-NA_R8-0346_2 | 18.8% | 52% | 207 bp | 0.9 | ||
| ENV_MELA_11_07-18-08_R8-1626 | 140.7Kbp | 4.8Mbp | ||||
| ENV_MELA_11_07-18-08_R8-1626_1 | 22.0% | 52% | 230 bp | 1.0 | ||
| ENV_MELA_11_07-18-08_R8-1626_2 | 19.9% | 52% | 219 bp | 1.0 | ||
| ENV_MELA_11_08-25-08_R8-2010 | 94.0Kbp | 4.8Mbp | ||||
| ENV_MELA_11_08-25-08_R8-2010_1 | 17.1% | 52% | 230 bp | 0.7 | ||
| ENV_MELA_11_08-25-08_R8-2010_2 | 12.9% | 52% | 200 bp | 0.7 | ||
| ENV_MELA_11_09-28-08_R8-2244 | 134.7Kbp | 4.8Mbp | ||||
| ENV_MELA_11_09-28-08_R8-2244_1 | 20.2% | 52% | 218 bp | 1.0 | ||
| ENV_MELA_11_09-28-08_R8-2244_2 | 17.5% | 52% | 202 bp | 1.0 | ||
| ENV_MELA_11_10-30-08_R8-2834 | 303.3Kbp | 4.8Mbp | ||||
| ENV_MELA_11_10-30-08_R8-2834_1 | 19.1% | 52% | 235 bp | 0.8 | ||
| ENV_MELA_11_10-30-08_R8-2834_2 | 17.7% | 52% | 224 bp | 0.8 | ||
| ENV_MELA_11_10-30-08_R8-2836 | 151.9Kbp | 4.8Mbp | ||||
| ENV_MELA_11_10-30-08_R8-2836_1 | 17.2% | 52% | 233 bp | 0.7 | ||
| ENV_MELA_11_10-30-08_R8-2836_2 | 15.5% | 52% | 220 bp | 0.7 | ||
| ENV_MELA_11_10-31-07_R6-0801 | 76.2Kbp | 4.8Mbp | ||||
| ENV_MELA_11_10-31-07_R6-0801_1 | 17.1% | 52% | 223 bp | 0.7 | ||
| ENV_MELA_11_10-31-07_R6-0801_2 | 14.9% | 52% | 207 bp | 0.7 | ||
| ENV_MINN_62_09-03-08_R8-2025 | 170.0Kbp | 4.5Mbp | ||||
| ENV_MINN_62_09-03-08_R8-2025_1 | 24.9% | 52% | 227 bp | 1.2 | ||
| ENV_MINN_62_09-03-08_R8-2025_2 | 23.0% | 52% | 217 bp | 1.2 | ||
| ENV_NEWP_17_02-12-08_R8-0341 | 352.0Kbp | 5.0Mbp | ||||
| ENV_NEWP_17_02-12-08_R8-0341_1 | 22.7% | 52% | 228 bp | 0.6 | ||
| ENV_NEWP_17_02-12-08_R8-0341_2 | 20.3% | 52% | 214 bp | 0.6 | ||
| ENV_NEWP_17_02-12-08_R8-0342 | 496.1Kbp | 5.0Mbp | ||||
| ENV_NEWP_17_02-12-08_R8-0342_1 | 23.2% | 52% | 222 bp | 1.0 | ||
| ENV_NEWP_17_02-12-08_R8-0342_2 | 22.6% | 52% | 217 bp | 1.0 | ||
| ENV_NEWP_35_07-23-08_R8-1638 | 115.6Kbp | 4.9Mbp | ||||
| ENV_NEWP_35_07-23-08_R8-1638_1 | 21.6% | 52% | 231 bp | 1.0 | ||
| ENV_NEWP_35_07-23-08_R8-1638_2 | 17.1% | 52% | 206 bp | 1.0 | ||
| ENV_NEWP_35_10-02-08_R8-2249 | 186.7Kbp | 4.9Mbp | ||||
| ENV_NEWP_35_10-02-08_R8-2249_1 | 23.2% | 52% | 214 bp | 1.1 | ||
| ENV_NEWP_35_10-02-08_R8-2249_2 | 13.6% | 51% | 166 bp | 1.1 | ||
| ENV_NEWP_35_11-05-08_R8-2843 | 55.6Kbp | 4.9Mbp | ||||
| ENV_NEWP_35_11-05-08_R8-2843_1 | 13.6% | 52% | 226 bp | 0.5 | ||
| ENV_NEWP_35_11-05-08_R8-2843_2 | 12.2% | 52% | 210 bp | 0.5 | ||
| ENV_NEWP_35_12-09-08_R8-3136 | 173.2Kbp | 4.9Mbp | ||||
| ENV_NEWP_35_12-09-08_R8-3136_1 | 24.2% | 51% | 202 bp | 1.1 | ||
| ENV_NEWP_35_12-09-08_R8-3136_2 | 11.7% | 51% | 146 bp | 1.1 | ||
| ENV_NEWP_62_01-22-09_R8-3410 | 113.7Kbp | 4.9Mbp | ||||
| ENV_NEWP_62_01-22-09_R8-3410_1 | 23.9% | 51% | 185 bp | 1.4 | ||
| ENV_NEWP_62_01-22-09_R8-3410_2 | 19.5% | 51% | 167 bp | 1.4 | ||
| ENV_NEWP_62_03-05-09_R8-3442 | 401.8Kbp | 4.9Mbp | ||||
| ENV_NEWP_62_03-05-09_R8-3442_1 | 25.3% | 51% | 200 bp | 1.3 | ||
| ENV_NEWP_62_03-05-09_R8-3442_2 | 20.3% | 51% | 179 bp | 1.3 | ||
| ENV_NEWP_62_05-28-09_R8-4026 | 128.6Kbp | 4.9Mbp | ||||
| ENV_NEWP_62_05-28-09_R8-4026_1 | 16.7% | 52% | 239 bp | 0.6 | ||
| ENV_NEWP_62_05-28-09_R8-4026_2 | 14.7% | 52% | 222 bp | 0.6 | ||
| ENV_NEWP_62_12-11-08_R8-3137 | 85.5Kbp | 4.9Mbp | ||||
| ENV_NEWP_62_12-11-08_R8-3137_1 | 18.9% | 52% | 208 bp | 0.9 | ||
| ENV_NEWP_62_12-11-08_R8-3137_2 | 16.4% | 52% | 192 bp | 0.9 | ||
| ENV_NEWP_62_12-11-08_R8-3139 | 344.8Kbp | 4.9Mbp | ||||
| ENV_NEWP_62_12-11-08_R8-3139_1 | 27.1% | 52% | 197 bp | 1.6 | ||
| ENV_NEWP_62_12-11-08_R8-3139_2 | 25.3% | 52% | 188 bp | 1.6 | ||
| ENV_TCOP_25_04-01-08_R8-0896 | 75.8Kbp | 5.1Mbp | ||||
| ENV_TCOP_25_04-01-08_R8-0896_1 | 22.5% | 52% | 218 bp | 1.3 | ||
| ENV_TCOP_25_04-01-08_R8-0896_2 | 21.6% | 52% | 211 bp | 1.3 | ||
| ENV_TCOP_25_04-01-08_R8-0898 | 48.2Kbp | 5.1Mbp | ||||
| ENV_TCOP_25_04-01-08_R8-0898_1 | 16.7% | 52% | 223 bp | 0.8 | ||
| ENV_TCOP_25_04-01-08_R8-0898_2 | 14.9% | 52% | 209 bp | 0.8 | ||
| ENV_TCOP_25_07-01-08_R8-1623 | 166.2Kbp | 5.1Mbp | ||||
| ENV_TCOP_25_07-01-08_R8-1623_1 | 10.0% | 52% | 231 bp | 0.4 | ||
| ENV_TCOP_25_07-01-08_R8-1623_2 | 9.3% | 52% | 222 bp | 0.4 | ||
| ENV_TCOP_25_07-29-08_R8-1642 | 73.5Kbp | 5.2Mbp | ||||
| ENV_TCOP_25_07-29-08_R8-1642_1 | 17.5% | 52% | 232 bp | 0.8 | ||
| ENV_TCOP_25_07-29-08_R8-1642_2 | 16.1% | 52% | 223 bp | 0.8 | ||
| ENV_TCOP_25_09-03-08_R8-2017 | 98.2Kbp | 5.1Mbp | ||||
| ENV_TCOP_25_09-03-08_R8-2017_1 | 19.6% | 52% | 229 bp | 0.9 | ||
| ENV_TCOP_25_09-03-08_R8-2017_2 | 17.8% | 52% | 217 bp | 0.9 | ||
| ENV_TYPH_22_01-18-08_R8-0174 | 178.4Kbp | 4.7Mbp | ||||
| ENV_TYPH_22_01-18-08_R8-0174_1 | 19.6% | 52% | 206 bp | 0.9 | ||
| ENV_TYPH_22_01-18-08_R8-0174_2 | 18.8% | 52% | 199 bp | 0.9 | ||
| ENV_TYPH_22_03-14-08_R8-0820 | 70.4Kbp | 4.7Mbp | ||||
| ENV_TYPH_22_03-14-08_R8-0820_1 | 19.7% | 52% | 220 bp | 1.0 | ||
| ENV_TYPH_22_03-14-08_R8-0820_2 | 18.4% | 52% | 211 bp | 1.0 | ||
| ENV_TYPH_22_12-17-07_R8-0171 | 145.3Kbp | 4.7Mbp | ||||
| ENV_TYPH_22_12-17-07_R8-0171_1 | 21.5% | 52% | 225 bp | 1.0 | ||
| ENV_TYPH_22_12-17-07_R8-0171_2 | 18.3% | 52% | 205 bp | 1.0 | ||
| ENV_TYPH_25_03-07-08_R8-0812 | 202.2Kbp | 5.0Mbp | ||||
| ENV_TYPH_25_03-07-08_R8-0812_1 | 19.2% | 52% | 225 bp | 0.9 | ||
| ENV_TYPH_25_03-07-08_R8-0812_2 | 17.5% | 52% | 215 bp | 0.9 | ||
| ENV_TYPH_25_05-01-08_R8-1385 | 11.9Kbp | 5.1Mbp | ||||
| ENV_TYPH_25_05-01-08_R8-1385_1 | 28.8% | 50% | 196 bp | 1.6 | ||
| ENV_TYPH_25_05-01-08_R8-1385_2 | 19.7% | 50% | 163 bp | 1.6 | ||
| ENV_TYPH_25_07-01-08_R8-1621 | 225.9Kbp | 5.1Mbp | ||||
| ENV_TYPH_25_07-01-08_R8-1621_1 | 17.3% | 52% | 208 bp | 0.8 | ||
| ENV_TYPH_25_07-01-08_R8-1621_2 | 16.0% | 52% | 200 bp | 0.8 | ||
| ENV_TYPH_25_07-01-08_R8-1622 | 225.9Kbp | 5.1Mbp | ||||
| ENV_TYPH_25_07-01-08_R8-1622_1 | 27.1% | 52% | 218 bp | 1.5 | ||
| ENV_TYPH_25_07-01-08_R8-1622_2 | 23.2% | 52% | 201 bp | 1.5 | ||
| ENV_TYPH_25_10-19-07_R6-0792 | 207.9Kbp | 4.9Mbp | ||||
| ENV_TYPH_25_10-19-07_R6-0792_1 | 20.8% | 51% | 228 bp | 1.0 | ||
| ENV_TYPH_25_10-19-07_R6-0792_2 | 18.4% | 51% | 214 bp | 1.0 | ||
| ENV_TYPH_25_10-19-07_R6-0793 | 191.7Kbp | 4.9Mbp | ||||
| ENV_TYPH_25_10-19-07_R6-0793_1 | 23.2% | 52% | 225 bp | 1.2 | ||
| ENV_TYPH_25_10-19-07_R6-0793_2 | 20.2% | 52% | 207 bp | 1.2 | ||
| ENV_TYPH_25_10-19-07_R6-0794 | 127.0Kbp | 4.9Mbp | ||||
| ENV_TYPH_25_10-19-07_R6-0794_1 | 15.0% | 52% | 218 bp | 0.6 | ||
| ENV_TYPH_25_10-19-07_R6-0794_2 | 14.3% | 52% | 209 bp | 0.6 |
QUAST
QUAST is a quality assessment tool for genome assemblies, written by the Center for Algorithmic Biotechnology.
Assembly Statistics
| Sample Name | N50 (Kbp) | N75 (Kbp) | L50 (K) | L75 (K) | Largest contig (Kbp) | Length (Mbp) |
|---|---|---|---|---|---|---|
| BOV_ANAT_39_02-21-08_R8-0842 | 59.3Kbp | 31.3Kbp | 0.0K | 50.0K | 264.8Kbp | 4.7Mbp |
| BOV_ANAT_39_02-21-08_R8-0847 | 77.6Kbp | 41.0Kbp | 0.0K | 42.0K | 173.9Kbp | 4.7Mbp |
| BOV_ANAT_56_04-19-08_R8-0968 | 95.6Kbp | 61.1Kbp | 0.0K | 32.0K | 262.7Kbp | 4.9Mbp |
| BOV_ANAT_56_04-19-08_R8-0969 | 251.4Kbp | 131.9Kbp | 0.0K | 16.0K | 396.8Kbp | 4.8Mbp |
| BOV_CERO_35_10-02-08_R8-2685 | 218.9Kbp | 132.4Kbp | 0.0K | 14.0K | 506.1Kbp | 4.7Mbp |
| BOV_CERO_62_11-12-08_R8-2903 | 268.8Kbp | 132.4Kbp | 0.0K | 13.0K | 622.5Kbp | 4.7Mbp |
| BOV_KENT_14_11-06-07_R6-0942 | 334.9Kbp | 107.1Kbp | 0.0K | 11.0K | 717.4Kbp | 4.7Mbp |
| BOV_KENT_14_12-13-07_R8-0083 | 234.0Kbp | 97.6Kbp | 0.0K | 16.0K | 513.9Kbp | 4.7Mbp |
| BOV_KENT_16_02-13-08_R8-0836 | 301.3Kbp | 149.0Kbp | 0.0K | 10.0K | 717.7Kbp | 4.7Mbp |
| BOV_KENT_16_02-13-08_R8-0838 | 218.9Kbp | 108.7Kbp | 0.0K | 16.0K | 408.6Kbp | 4.7Mbp |
| BOV_KENT_16_04-03-08_R8-0967 | 167.9Kbp | 59.4Kbp | 0.0K | 22.0K | 440.0Kbp | 4.7Mbp |
| BOV_KENT_16_05-21-08_R8-1485 | 484.7Kbp | 163.1Kbp | 0.0K | 8.0K | 745.8Kbp | 4.7Mbp |
| BOV_KENT_17_02-12-08_R8-0823 | 244.9Kbp | 148.3Kbp | 0.0K | 11.0K | 717.7Kbp | 4.7Mbp |
| BOV_KENT_17_11-07-07_R6-0957 | 250.0Kbp | 148.6Kbp | 0.0K | 10.0K | 718.0Kbp | 4.7Mbp |
| BOV_KENT_17_12-12-07_R8-0211 | 161.5Kbp | 88.6Kbp | 0.0K | 21.0K | 346.0Kbp | 4.7Mbp |
| BOV_KENT_19_11-19-07_R6-0966 | 244.9Kbp | 157.7Kbp | 0.0K | 12.0K | 515.0Kbp | 4.7Mbp |
| BOV_KENT_42_03-13-08_R8-0863 | 408.3Kbp | 218.9Kbp | 0.0K | 8.0K | 745.8Kbp | 4.8Mbp |
| BOV_MELA_01_10-02-07_R6-0951 | 244.9Kbp | 209.3Kbp | 0.0K | 11.0K | 682.8Kbp | 4.8Mbp |
| BOV_MELA_01_10-30-07_R6-0802 | 336.5Kbp | 215.8Kbp | 0.0K | 9.0K | 712.0Kbp | 4.8Mbp |
| BOV_MELA_01_10-30-07_R6-0803 | 207.1Kbp | 116.8Kbp | 0.0K | 16.0K | 455.4Kbp | 4.8Mbp |
| BOV_MELA_01_10-30-07_R6-0810 | 208.4Kbp | 103.5Kbp | 0.0K | 15.0K | 795.7Kbp | 4.8Mbp |
| BOV_MELA_01_12-11-07_R8-0198 | 240.4Kbp | 192.2Kbp | 0.0K | 13.0K | 616.1Kbp | 4.8Mbp |
| BOV_NEWP_17_11-07-07_R6-0956 | 172.3Kbp | 96.3Kbp | 0.0K | 20.0K | 341.6Kbp | 5.0Mbp |
| BOV_NEWP_17_12-12-07_R8-0206 | 300.2Kbp | 145.6Kbp | 0.0K | 12.0K | 849.1Kbp | 5.0Mbp |
| BOV_NEWP_35_10-02-08_R8-2688 | 503.8Kbp | 380.8Kbp | 0.0K | 6.0K | 1251.1Kbp | 4.9Mbp |
| BOV_NEWP_62_10-14-08_R8-2874 | 496.1Kbp | 260.4Kbp | 0.0K | 7.0K | 1251.3Kbp | 4.9Mbp |
| BOV_NEWP_62_11-12-08_R8-2900 | 504.2Kbp | 380.7Kbp | 0.0K | 6.0K | 1251.3Kbp | 4.9Mbp |
| BOV_TYPH_17_12-12-07_R8-0208 | 191.7Kbp | 114.0Kbp | 0.0K | 15.0K | 430.4Kbp | 4.7Mbp |
| BOV_TYPH_22_01-18-08_R8-0227 | 214.2Kbp | 128.3Kbp | 0.0K | 14.0K | 719.8Kbp | 4.7Mbp |
| BOV_TYPH_22_01-18-08_R8-0228 | 107.9Kbp | 58.5Kbp | 0.0K | 26.0K | 388.9Kbp | 4.7Mbp |
| BOV_TYPH_22_01-18-08_R8-0229 | 75.4Kbp | 45.3Kbp | 0.0K | 40.0K | 223.3Kbp | 4.8Mbp |
| BOV_TYPH_22_01-18-08_R8-0231 | 95.1Kbp | 63.5Kbp | 0.0K | 29.0K | 329.4Kbp | 4.7Mbp |
| BOV_TYPH_22_01-18-08_R8-0271 | 275.5Kbp | 149.9Kbp | 0.0K | 12.0K | 719.8Kbp | 4.7Mbp |
| BOV_TYPH_22_01-18-08_R8-0273 | 187.9Kbp | 91.0Kbp | 0.0K | 15.0K | 719.8Kbp | 4.7Mbp |
| BOV_TYPH_22_03-14-08_R8-0865 | 216.2Kbp | 107.5Kbp | 0.0K | 15.0K | 719.8Kbp | 4.9Mbp |
| BOV_TYPH_25_01-15-08_R8-0233 | 126.2Kbp | 80.3Kbp | 0.0K | 27.0K | 361.6Kbp | 5.0Mbp |
| BOV_TYPH_25_11-20-07_R6-0978 | 143.3Kbp | 74.6Kbp | 0.0K | 25.0K | 292.1Kbp | 5.0Mbp |
| BOV_TYPH_25_11-20-07_R6-0979 | 266.9Kbp | 149.7Kbp | 0.0K | 14.0K | 512.4Kbp | 4.9Mbp |
| BOV_TYPH_25_11-20-07_R6-0983 | 81.8Kbp | 42.4Kbp | 0.0K | 39.0K | 199.5Kbp | 5.0Mbp |
| ENV_ANAT_39_01-09-08_R8-0187 | 403.1Kbp | 272.8Kbp | 0.0K | 7.0K | 1093.8Kbp | 4.7Mbp |
| ENV_ANAT_39_02-21-08_R8-0348 | 209.1Kbp | 93.0Kbp | 0.0K | 16.0K | 763.8Kbp | 4.7Mbp |
| ENV_ANAT_39_04-17-08_R8-0905 | 468.8Kbp | 375.1Kbp | 0.0K | 6.0K | 1496.7Kbp | 4.7Mbp |
| ENV_ANAT_39_11-13-07_R6-0928 | 91.1Kbp | 47.3Kbp | 0.0K | 35.0K | 268.2Kbp | 4.7Mbp |
| ENV_ANAT_39_11-13-07_R6-0929 | 240.8Kbp | 107.1Kbp | 0.0K | 15.0K | 632.5Kbp | 4.8Mbp |
| ENV_ANAT_39_12-03-08_R8-2860 | 387.1Kbp | 209.2Kbp | 0.0K | 9.0K | 904.4Kbp | 4.7Mbp |
| ENV_ANAT_56_02-22-08_R8-0350 | 159.5Kbp | 81.8Kbp | 0.0K | 21.0K | 380.5Kbp | 4.8Mbp |
| ENV_ANAT_56_03-25-09_R8-3652 | 169.7Kbp | 112.2Kbp | 0.0K | 19.0K | 363.5Kbp | 4.7Mbp |
| ENV_ANAT_56_03-25-09_R8-3653 | 200.0Kbp | 108.1Kbp | 0.0K | 16.0K | 507.9Kbp | 4.8Mbp |
| ENV_ANAT_56_04-18-08_R8-0907 | 305.7Kbp | 137.0Kbp | 0.0K | 12.0K | 571.4Kbp | 4.8Mbp |
| ENV_ANAT_56_06-12-08_R8-1402 | 415.4Kbp | 328.9Kbp | 0.0K | 7.0K | 806.4Kbp | 4.8Mbp |
| ENV_CERO_62_01-22-09_R8-3412 | 196.6Kbp | 95.4Kbp | 0.0K | 17.0K | 572.6Kbp | 4.7Mbp |
| ENV_CERO_62_03-05-09_R8-3445 | 166.9Kbp | 81.9Kbp | 0.0K | 21.0K | 344.0Kbp | 4.7Mbp |
| ENV_CERO_62_04-16-09_R8-3664 | 131.4Kbp | 59.1Kbp | 0.0K | 24.0K | 323.6Kbp | 4.7Mbp |
| ENV_CERO_62_05-28-09_R8-4025 | 69.4Kbp | 33.8Kbp | 0.0K | 45.0K | 247.1Kbp | 4.7Mbp |
| ENV_CERO_62_07-09-09_R8-4030 | 142.8Kbp | 77.2Kbp | 0.0K | 21.0K | 321.8Kbp | 4.7Mbp |
| ENV_CERO_62_07-22-08_R8-1631 | 168.7Kbp | 81.9Kbp | 0.0K | 21.0K | 433.8Kbp | 4.7Mbp |
| ENV_CERO_62_07-22-08_R8-1632 | 213.2Kbp | 120.4Kbp | 0.0K | 15.0K | 549.9Kbp | 4.7Mbp |
| ENV_CERO_62_09-03-08_R8-2026 | 222.6Kbp | 139.7Kbp | 0.0K | 13.0K | 622.9Kbp | 4.7Mbp |
| ENV_CERO_62_10-14-08_R8-2818 | 222.6Kbp | 142.8Kbp | 0.0K | 13.0K | 622.5Kbp | 4.7Mbp |
| ENV_CERO_62_11-12-08_R8-2852 | 210.3Kbp | 132.4Kbp | 0.0K | 15.0K | 412.3Kbp | 4.7Mbp |
| ENV_CERO_62_12-11-08_R8-3138 | 213.4Kbp | 95.4Kbp | 0.0K | 15.0K | 622.5Kbp | 4.7Mbp |
| ENV_KENT_14_02-14-08_R8-0344 | 274.4Kbp | 127.9Kbp | 0.0K | 12.0K | 717.9Kbp | 4.7Mbp |
| ENV_KENT_14_08-30-08_R8-2016 | 247.8Kbp | 104.4Kbp | 0.0K | 14.0K | 633.6Kbp | 4.7Mbp |
| ENV_KENT_14_10-11-07_R6-0798 | 144.5Kbp | 71.8Kbp | 0.0K | 23.0K | 259.3Kbp | 4.7Mbp |
| ENV_KENT_16_09-09-08_R8-2031 | 197.7Kbp | 76.0Kbp | 0.0K | 19.0K | 633.6Kbp | 4.7Mbp |
| ENV_KENT_16_11-11-08_R8-2848 | 247.7Kbp | 76.0Kbp | 0.0K | 17.0K | 642.5Kbp | 4.7Mbp |
| ENV_KENT_16_12-04-07_R8-0061 | 29.8Kbp | 18.9Kbp | 0.0K | 92.0K | 192.2Kbp | 4.7Mbp |
| ENV_KENT_17_03-11-08_R8-0814 | 247.7Kbp | 163.1Kbp | 0.0K | 11.0K | 633.6Kbp | 4.7Mbp |
| ENV_KENT_17_03-11-08_R8-0815 | 76.6Kbp | 37.6Kbp | 0.0K | 42.0K | 218.9Kbp | 4.8Mbp |
| ENV_KENT_17_06-17-08_R8-1405 | 265.7Kbp | 218.9Kbp | 0.0K | 10.0K | 745.8Kbp | 4.7Mbp |
| ENV_KENT_17_07-27-08_R8-1641 | 97.9Kbp | 51.4Kbp | 0.0K | 33.0K | 278.8Kbp | 4.7Mbp |
| ENV_KENT_17_09-10-08_R8-2033 | 89.8Kbp | 59.0Kbp | 0.0K | 33.0K | 249.1Kbp | 4.7Mbp |
| ENV_KENT_17_10-11-07_R6-1000 | 97.8Kbp | 62.2Kbp | 0.0K | 27.0K | 287.4Kbp | 4.7Mbp |
| ENV_KENT_19_01-16-08_R8-0168 | 244.9Kbp | 127.3Kbp | 0.0K | 12.0K | 642.6Kbp | 4.7Mbp |
| ENV_KENT_19_03-13-08_R8-0817 | 107.7Kbp | 71.3Kbp | 0.0K | 27.0K | 328.7Kbp | 4.7Mbp |
| ENV_KENT_19_04-28-08_R8-0916 | 250.1Kbp | 128.2Kbp | 0.0K | 11.0K | 717.9Kbp | 4.7Mbp |
| ENV_KENT_19_04-28-08_R8-0917 | 265.8Kbp | 218.9Kbp | 0.0K | 10.0K | 745.8Kbp | 4.7Mbp |
| ENV_KENT_19_07-21-08_R8-1644 | 205.5Kbp | 77.5Kbp | 0.0K | 21.0K | 377.2Kbp | 4.7Mbp |
| ENV_KENT_19_10-02-08_R8-2813 | 127.9Kbp | 64.5Kbp | 0.0K | 27.0K | 312.6Kbp | 4.7Mbp |
| ENV_KENT_42_01-17-08_R8-0191 | 191.5Kbp | 97.5Kbp | 0.0K | 18.0K | 506.0Kbp | 4.7Mbp |
| ENV_KENT_42_04-23-08_R8-0913 | 433.4Kbp | 237.5Kbp | 0.0K | 8.0K | 745.8Kbp | 4.7Mbp |
| ENV_KENT_42_04-23-08_R8-0914 | 484.6Kbp | 163.1Kbp | 0.0K | 8.0K | 745.8Kbp | 4.7Mbp |
| ENV_KENT_42_07-23-08_R8-1640 | 150.2Kbp | 76.0Kbp | 0.0K | 22.0K | 368.6Kbp | 4.7Mbp |
| ENV_KENT_42_08-19-08_R8-2009 | 197.8Kbp | 102.7Kbp | 0.0K | 17.0K | 435.8Kbp | 4.7Mbp |
| ENV_KENT_42_09-17-08_R8-2236 | 206.4Kbp | 89.0Kbp | 0.0K | 18.0K | 484.6Kbp | 4.7Mbp |
| ENV_KENT_42_11-15-07_R6-0935 | 228.1Kbp | 148.7Kbp | 0.0K | 12.0K | 745.8Kbp | 4.7Mbp |
| ENV_MELA_01_01-10-08_R8-0165 | 211.8Kbp | 105.0Kbp | 0.0K | 16.0K | 550.7Kbp | 4.8Mbp |
| ENV_MELA_01_04-04-08_R8-0901 | 173.8Kbp | 62.7Kbp | 0.0K | 23.0K | 366.3Kbp | 4.8Mbp |
| ENV_MELA_01_05-08-08_R8-1381 | 411.2Kbp | 158.7Kbp | 0.0K | 9.0K | 641.2Kbp | 4.8Mbp |
| ENV_MELA_01_06-13-08_R8-1406 | 492.5Kbp | 265.1Kbp | 0.0K | 7.0K | 927.7Kbp | 4.8Mbp |
| ENV_MELA_01_07-23-08_R8-1633 | 173.0Kbp | 92.3Kbp | 0.0K | 19.0K | 429.6Kbp | 4.8Mbp |
| ENV_MELA_01_09-10-08_R8-2233 | 410.6Kbp | 169.0Kbp | 0.0K | 9.0K | 927.7Kbp | 4.8Mbp |
| ENV_MELA_01_10-02-07_R6-0938 | 217.8Kbp | 106.8Kbp | 0.0K | 15.0K | 700.7Kbp | 4.9Mbp |
| ENV_MELA_01_10-22-08_R8-2829 | 207.1Kbp | 107.3Kbp | 0.0K | 16.0K | 428.8Kbp | 4.8Mbp |
| ENV_MELA_01_NA-NA-NA_R8-0346 | 240.4Kbp | 133.2Kbp | 0.0K | 13.0K | 693.7Kbp | 4.8Mbp |
| ENV_MELA_11_07-18-08_R8-1626 | 140.7Kbp | 74.4Kbp | 0.0K | 24.0K | 278.7Kbp | 4.8Mbp |
| ENV_MELA_11_08-25-08_R8-2010 | 94.0Kbp | 47.2Kbp | 0.0K | 31.0K | 371.3Kbp | 4.8Mbp |
| ENV_MELA_11_09-28-08_R8-2244 | 134.7Kbp | 74.3Kbp | 0.0K | 24.0K | 280.3Kbp | 4.8Mbp |
| ENV_MELA_11_10-30-08_R8-2834 | 303.3Kbp | 158.0Kbp | 0.0K | 12.0K | 616.4Kbp | 4.8Mbp |
| ENV_MELA_11_10-30-08_R8-2836 | 151.9Kbp | 81.9Kbp | 0.0K | 18.0K | 787.3Kbp | 4.8Mbp |
| ENV_MELA_11_10-31-07_R6-0801 | 76.2Kbp | 49.2Kbp | 0.0K | 38.0K | 244.9Kbp | 4.8Mbp |
| ENV_MINN_62_09-03-08_R8-2025 | 170.0Kbp | 110.8Kbp | 0.0K | 18.0K | 412.8Kbp | 4.5Mbp |
| ENV_NEWP_17_02-12-08_R8-0341 | 352.0Kbp | 177.3Kbp | 0.0K | 9.0K | 921.7Kbp | 5.0Mbp |
| ENV_NEWP_17_02-12-08_R8-0342 | 496.1Kbp | 177.2Kbp | 0.0K | 7.0K | 1100.0Kbp | 5.0Mbp |
| ENV_NEWP_35_07-23-08_R8-1638 | 115.6Kbp | 54.2Kbp | 0.0K | 28.0K | 310.3Kbp | 4.9Mbp |
| ENV_NEWP_35_10-02-08_R8-2249 | 186.7Kbp | 78.2Kbp | 0.0K | 20.0K | 383.3Kbp | 4.9Mbp |
| ENV_NEWP_35_11-05-08_R8-2843 | 55.6Kbp | 28.7Kbp | 0.0K | 58.0K | 221.6Kbp | 4.9Mbp |
| ENV_NEWP_35_12-09-08_R8-3136 | 173.2Kbp | 80.2Kbp | 0.0K | 22.0K | 379.8Kbp | 4.9Mbp |
| ENV_NEWP_62_01-22-09_R8-3410 | 113.7Kbp | 74.0Kbp | 0.0K | 28.0K | 375.3Kbp | 4.9Mbp |
| ENV_NEWP_62_03-05-09_R8-3442 | 401.8Kbp | 159.7Kbp | 0.0K | 10.0K | 1173.1Kbp | 4.9Mbp |
| ENV_NEWP_62_05-28-09_R8-4026 | 128.6Kbp | 58.9Kbp | 0.0K | 27.0K | 323.4Kbp | 4.9Mbp |
| ENV_NEWP_62_12-11-08_R8-3137 | 85.5Kbp | 45.2Kbp | 0.0K | 38.0K | 220.9Kbp | 4.9Mbp |
| ENV_NEWP_62_12-11-08_R8-3139 | 344.8Kbp | 89.7Kbp | 0.0K | 13.0K | 1103.5Kbp | 4.9Mbp |
| ENV_TCOP_25_04-01-08_R8-0896 | 75.8Kbp | 41.0Kbp | 0.0K | 48.0K | 244.6Kbp | 5.1Mbp |
| ENV_TCOP_25_04-01-08_R8-0898 | 48.2Kbp | 25.9Kbp | 0.0K | 66.0K | 192.3Kbp | 5.1Mbp |
| ENV_TCOP_25_07-01-08_R8-1623 | 166.2Kbp | 91.2Kbp | 0.0K | 17.0K | 608.3Kbp | 5.1Mbp |
| ENV_TCOP_25_07-29-08_R8-1642 | 73.5Kbp | 36.6Kbp | 0.0K | 48.0K | 246.7Kbp | 5.2Mbp |
| ENV_TCOP_25_09-03-08_R8-2017 | 98.2Kbp | 47.7Kbp | 0.0K | 32.0K | 340.4Kbp | 5.1Mbp |
| ENV_TYPH_22_01-18-08_R8-0174 | 178.4Kbp | 92.9Kbp | 0.0K | 18.0K | 430.2Kbp | 4.7Mbp |
| ENV_TYPH_22_03-14-08_R8-0820 | 70.4Kbp | 37.8Kbp | 0.0K | 44.0K | 191.7Kbp | 4.7Mbp |
| ENV_TYPH_22_12-17-07_R8-0171 | 145.3Kbp | 65.2Kbp | 0.0K | 24.0K | 429.6Kbp | 4.7Mbp |
| ENV_TYPH_25_03-07-08_R8-0812 | 202.2Kbp | 81.6Kbp | 0.0K | 17.0K | 460.6Kbp | 5.0Mbp |
| ENV_TYPH_25_05-01-08_R8-1385 | 11.9Kbp | 5.7Kbp | 0.1K | 284.0K | 54.0Kbp | 5.1Mbp |
| ENV_TYPH_25_07-01-08_R8-1621 | 225.9Kbp | 91.6Kbp | 0.0K | 16.0K | 659.2Kbp | 5.1Mbp |
| ENV_TYPH_25_07-01-08_R8-1622 | 225.9Kbp | 91.6Kbp | 0.0K | 16.0K | 659.7Kbp | 5.1Mbp |
| ENV_TYPH_25_10-19-07_R6-0792 | 207.9Kbp | 91.0Kbp | 0.0K | 16.0K | 453.5Kbp | 4.9Mbp |
| ENV_TYPH_25_10-19-07_R6-0793 | 191.7Kbp | 74.3Kbp | 0.0K | 20.0K | 453.5Kbp | 4.9Mbp |
| ENV_TYPH_25_10-19-07_R6-0794 | 127.0Kbp | 61.5Kbp | 0.0K | 27.0K | 275.5Kbp | 4.9Mbp |
Number of Contigs
This plot shows the number of contigs found for each assembly, broken down by length.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
FastQC
FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.
Sequence Counts
Sequence counts for each sample. Duplicate read counts are an estimate only.
This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).
You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:
Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.
The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Sequence Quality Histograms
The mean quality value across each base position in the read.
To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).
Taken from the FastQC help:
The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Per Sequence Quality Scores
The number of reads with average quality scores. Shows if a subset of reads has poor quality.
From the FastQC help:
The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Per Base Sequence Content
The proportion of each base position for which each of the four normal DNA bases has been called.
To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.
To see the data as a line plot, as in the original FastQC graph, click on a sample track.
From the FastQC help:
Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.
In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.
It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.
Rollover for sample name
Per Sequence GC Content
The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.
From the FastQC help:
This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.
In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.
An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Per Base N Content
The percentage of base calls at each position for which an N was called.
From the FastQC help:
If a sequencer is unable to make a base call with sufficient confidence then it will
normally substitute an N rather than a conventional base call. This graph shows the
percentage of base calls at each position for which an N was called.
It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Sequence Length Distribution
The distribution of fragment sizes (read lengths) found. See the FastQC help
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Sequence Duplication Levels
The relative level of duplication found for every sequence.
From the FastQC Help:
In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.
Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.
The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.
In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.
Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).
Overrepresented sequences
The total amount of overrepresented sequences found in each library.
FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.
Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.
From the FastQC Help:
A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.
FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.
Adapter Content
The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.
Note that only samples with ≥ 0.1% adapter contamination are shown.
There may be several lines per sample, as one is shown for each adapter detected in the file.
From the FastQC Help:
The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.
Status Checks
Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).
FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).
It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.
Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.
In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.
